Lymphocyte function in patients with certain neurological diseases

Menzies, Catherine B (1980) Lymphocyte function in patients with certain neurological diseases. MSc(R) thesis, University of Glasgow.

Abstract

The quantitative evaluation of the reactivity in human phytohaemagglutinin-stimulated lymphocyte cultures was studied using a whole blood technique. Cells were cultured for 22 hours in various concentrations of PHA, and by measuring the amount of incorporation of tritium labelled leucine into the proteins synthesised, a dose response curve could be drawn up for each donor. The patients studied fell into three main categories, (a) 22 patients with brain tumours. Depressed protein synthesis was detected in many of these when compared to 35 normal healthy controls, and also to 20 patients with various chronic neurological diseases. A 3-day culture technique was developed to test the effect of sera from patients with brain tumours, and other neurological complaints, on normal lymphocytes. No serum blocking factor was found. (b) Patients undergoing practolol therapy. Impaired T-cell function was seen in all patients whether they manifested adverse reactions to the drug in vivo or not. Normal lymphocytes were cultured for 2 days in the presence of various beta-blocking drugs, then their response to PHA was measured. Only propranolol was seen to cause depression of protein synthesis, (c) 31 patients with epilepsy who were taking various anticonvulsant drugs, including phenytoin. Low responses were seen in all patients on anticonvulsants when compared to 35 normal healthy controls.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Keywords: Neurosciences
• Colleges/Schools: College of Medical Veterinary and Life Sciences
• Supervisor's Name: Newth, Professor D.R.
• Date of Award: 1980
• Depositing User: Enlighten Team
• Unique ID: glathesis:1980-74172
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 23 Sep 2019 15:33
• Last Modified: 15 Aug 2022 10:14
• Thesis DOI: 10.5525/gla.thesis.74172
• URI: https://theses.gla.ac.uk/id/eprint/74172