The Effect of Different TALP-Components on the Progressive Motility of Bovine Spermatozoa During the Centrifugation and Incubation Stages as a Prerequisite for In Vitro Fertilisation

Kinis, Athanasios K (1988) The Effect of Different TALP-Components on the Progressive Motility of Bovine Spermatozoa During the Centrifugation and Incubation Stages as a Prerequisite for In Vitro Fertilisation. MVM(R) thesis, University of Glasgow.

Abstract

In the present study the effect of different Modified Tyrode's media-TALP and different sperm concentrations on the progressive motility of the spermatozoa was examined. TALP medium containing high levels of lactate, NaHCO3 and Hepes provided the best environment for the spermatozoa during the swim-up first and second centrifugation stages. However the medium, in which lactate was the primary nutritional source, gave the best results during CO2 incubation. Treatments of spermatozoa in an air environment gave much poor results than treatments in a CO2 environment. Sperm motility was optimal when the sperm concentration was around 20x10.

• Item Type: Thesis (MVM(R))
• Qualification Level: Masters
• Keywords: Veterinary science
• Date of Award: 1988
• Depositing User: Enlighten Team
• Unique ID: glathesis:1988-77687
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77687