McNamara, Laura Elizabeth (2010) Molecular and cellular analysis of topography-induced mechanotransduction. PhD thesis, University of Glasgow.Due to Embargo and/or Third Party Copyright restrictions, this thesis is not available in this service.
Edited Abstract: Mechanotransduction is the process by which cells convert mechanical stimuli into an adaptive gene- and protein-level response, via signalling cascades or direct physical effects of the cytoskeleton on the nucleus, and appropriate mechanosignalling is crucial for tissue development and function. Most techniques currently used to study cellular mechanoresponses are relatively damaging to the cells. In contrast, topographically structured substrates, such as microgrooves, have great potential for use as non-invasive mechanostimuli. In this study, quartz microgrooved substrata (2 μm depth x 25 μm pitch) were used as platforms for the confinement and alignment of cells. A multi-layered approach was adopted to begin to integrate the changes induced by the topographical mechanostimulus at the chromosome, small RNA, transcript, protein and structural levels. Together, the results provide insight into multiple facets of topography-induced mechanotransduction, which should contribute to understanding of mechanotransduction and cell-material interactions.
|Item Type:||Thesis (PhD)|
|Keywords:||Mechanotransduction, microtopography, biomaterials, cell biology, cell engineering, molecular biology, proteomics, transcriptomics|
|Subjects:||R Medicine > R Medicine (General)
Q Science > Q Science (General)
|Colleges/Schools:||College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation|
|Supervisor's Name:||Dalby, Dr. Matthew J. and Riehle, Dr. Mathis O. and Burchmore, Dr. Richard|
|Date of Award:||2010|
|Embargo Date:||22 October 2013|
|Depositing User:||Ms Laura E McNamara|
|Copyright:||Copyright of this thesis is held by the author.|
|Date Deposited:||04 Nov 2010|
|Last Modified:||10 Dec 2012 13:52|
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