Facing disease challenges through novel approaches in molecular biology, epidemiology and cell biochemistry

Casey, Miriam (2011) Facing disease challenges through novel approaches in molecular biology, epidemiology and cell biochemistry. MSc(R) thesis, University of Glasgow.

Due to Embargo and/or Third Party Copyright restrictions, this thesis is not available in this service.

Abstract

1. Haemonchus contortus, a parasitic nematode of small ruminants presents significant problems to the international farming industry which are confounded by multi-anthelminthic resistance. Better understanding of this parasite and its developmental requirements are a priority for the development of novel therapeutic agents. Parasite specific microRNAs (small non-coding RNAs which regulate gene expression) are potential future anthelminthic targets. However, little is known about microRNAs regulatory systems in parasitic nematodes. Here, we aimed to examine whether H. contortus expressed lin-41, a mRNA target for let-7 microRNA. H. contortus lin-41 (Hc-lin-41) was amplified and sequenced, as was its 3′ untranslated region (3′ UTR). Six potential let-7 binding sites were identified bioinformatically in the 3′ UTR of Hc-lin-41. It can be concluded from this that the regulation of lin-41 mRNA by let-7 microRNA is a valid avenue for further research in H. contortus.

Item Type:	Thesis (MSc(R))
Qualification Level:	Masters
Subjects:	S Agriculture > SF Animal culture > SF600 Veterinary Medicine
Colleges/Schools:	College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Supervisor's Name:	McLaughlin, Dr. Mark and Britton, Dr. Colette and Boden, Dr. Lisa and Devaney, Prof. Eileen
Date of Award:	2011
Embargo Date:	9 February 2015
Depositing User:	Mrs Marie Cairney
Unique ID:	glathesis:2011-3186
Copyright:	Copyright of this thesis is held by the author.
Date Deposited:	10 Feb 2012
Last Modified:	10 Dec 2012 14:04
URI:	http://theses.gla.ac.uk/id/eprint/3186

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