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Optimisation of interphase fluorescence in situ hybridisation for detection of common aneuploidies

Mohaddes Ardebili, Seyed Mojtaba (1996) Optimisation of interphase fluorescence in situ hybridisation for detection of common aneuploidies. PhD thesis, University of Glasgow.

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Abstract

The optimisation of a simple, reliable and practical method of interphase FISH which allows prenatal diagnosis of major chromosome aneuploidies using a minimum volume of amniotic fluid sample was the overall objective of this study. When all the probes required were available, the study continued by developing the technique of ratio-mixing FISH for simultaneous detection of the five major chromosome aneuploidies. The technique of five-colour ratio mixing FISH which has been presented here is simple and straightforward, since only two haptenisation and detection systems have been employed to visualise simultaneously five different targets in five distinguishable colours. The steps of denaturation, hybridisation and detection are the same as those used in a uni-colour FISH experiment. The results obtained from hybridisation of an unselected series of 20 uncultured lymphocytes and 27 uncultured amniocytes indicate that the technique is reliable and can be used for simultaneous detection of major chromosome aneuploidies. In order to provide a practical strategy for clinical diagnostic purposes, the use of a three colour ratio-mixing FISH and a dual colour was investigated to visualise the five probe sets on two slides from the same sample. A total unselected series of 45 uncultured lymphocytes and 60 uncultured aminocytes were hybridised with different probe combinations using three colour ratio-mixing FISH. The results indicate that the major chromosome aneuploidies can be simply and reliably identified on two slides from the same sample, using a three colour ratio-mixing FISH to detect the chromosomes X, Y and 21 and a dual colour to detect chromosomes 13 and 18. The failure rate was reduced to 4 per cent using this approach.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Subjects: UNSPECIFIED
Colleges/Schools: College of Medical Veterinary and Life Sciences
Supervisor's Name: Boyd, Dr. Elizabeth
Date of Award: 1996
Depositing User: Geraldine Coyle
Unique ID: glathesis:1996-692
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 15 Apr 2009
Last Modified: 10 Dec 2012 13:24
URI: http://theses.gla.ac.uk/id/eprint/692

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