Human embryonic stem cell-derived mesenchymal stem cells as a therapy for spinal cord injury

Che Mohamad, Che Anuar (2014) Human embryonic stem cell-derived mesenchymal stem cells as a therapy for spinal cord injury. PhD thesis, University of Glasgow.

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Abstract

Traumatic injury to the spinal cord interrupts ascending and descending pathways leading to severe functional deficits of sensory motor and autonomic function which depend on the level and severity of the injury. There are currently no effective therapies for treating such injuries and the adult central nervous system has very limited capacity for repair so that recovery is very limited and functional deficits are usually permanent. Cell transplantation is a potential therapy for spinal cord injury and a range of cell types are being investigated as candidates. Mesenchymal stem cells (MSCs) obtained from bone marrow are one cell type quite extensively studied. When transplanted into animal models of spinal cord injury these cells are reported to affect various aspects of repair and in some cases to improve functional outcome according to behavioural measures. However, the use of these cells has several limitations including the need for an invasive harvesting procedure, variability in cell quality and slow expansion in culture. This project therefore had two main aims: Firstly to investigate whether MSC-like cells closely equivalent to bone marrow derived MSCs could be reliably and consistently differentiated from human embryonic stem cells (hESCs) in order to provide an “off the shelf” cellular therapy product for spinal cord injury and secondly, to transplant such cells into animal models of spinal cord injury in order to, determine whether hESC-derived MSCs replicate or improve on the repair mechanisms reported for bone marrow MSCs.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Subjects: Q Science > QR Microbiology
R Medicine > RC Internal medicine
Colleges/Schools: College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Funder's Name: UNSPECIFIED
Supervisor's Name: Riddell, Dr. J.
Date of Award: 2014
Depositing User: Mrs Marie Cairney
Unique ID: glathesis:2014-7047
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 26 Jan 2016 09:49
Last Modified: 26 Jan 2016 10:04
URI: http://theses.gla.ac.uk/id/eprint/7047

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