Investigations on the control of cell behaviour and the cell cycle

Tripathi, Satish Chandra (1984) Investigations on the control of cell behaviour and the cell cycle. PhD thesis, University of Glasgow.

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Abstract

Little work has been done previously on cells grown as sail-sheets. This thesis describes the morphology, behaviour and movement of chick heart fibroblasts (CHFs) in sail-sheets and the effects of mechanical tension on actin content and the cell cycle of these cells. Abercrombie et ad. (1970 a) quantified the measurements on the features of movement of CHFs on glass coverslips (or, conventional cultures). Since the sail-sheet cultures appeared to resemble more closely the in vivo situation than the conventional cultures, it seemed appropriate that the features of cell movement in sail-sheets be studied and compared with those in conventional cultures. The work presented in this thesis suggests that CHFs in sail-sheets do exhibit such features as described for conventional cultures (Abercrombie et al., 1970 a) but at a relatively lower speed. Mechanical tension occurs within and between cells during embryogenesis, wound healing and in the repetitive contractile processes performed by various muscles of the body. Curtis and Seehar (1978) found that short-term tensing of sail-sheets with a low frequency oscillator shortened the duration of the cell cycle in CHFs. This thesis investigates whether tensing of sail-sheets for longer durations and at much lower frequencies produces any different effects than those found by Curtis and Seehar (1978). The work from these experiments suggested that on the whole, tension causes a reduction in the duration of the cell cycle. The effects of tension in a rectangular cell sheet differ from corners, edges and centres perhaps because of local stress concentration. The hypothesis that the effect of tension on the cell cycle may be due to its effect on the microfilaments was investigated. Results were inconclusive.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: A S G Curtis
Keywords: Biomedical engineering
Date of Award: 1984
Depositing User: Enlighten Team
Unique ID: glathesis:1984-71591
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 10 May 2019 14:10
Last Modified: 10 May 2019 14:10
URI: http://theses.gla.ac.uk/id/eprint/71591

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