Studies on cell adhesion in relation to immune responses

Gallagher, Richard B (1984) Studies on cell adhesion in relation to immune responses. PhD thesis, University of Glasgow.

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Abstract

This work can be divided into three sections; the effects (i) of H-2D antigens on peritoneal exudate cell (PEC) adherence, (ii) of la antigens on PEC and lymphocytes and (iii) of reactive oxygen intermediates on lymphocyte activation. H-2D antigen fragments can be attached to glass substates in an antigenically recognizable form and can affect the adhesion of PEC to the substrate. In all cases a significantly larger number (by t-test) of syngeneic than allogeneic cells adhere to fragments of the b, d and k allotypes. No differences between allogeneic and syngeneic adhesion could be found by interference reflection microscopy. When the average spread areas of cells were measured, they were found to be greater for allogeneic than for syngeneic combinations, although this could be due to contact inhibition of spreading in the latter. This work supports the suggestion that the MHC is involved in the modulation of cell adhesion. The incubation of PEC in the supernatant of con A stimulated spleen cells resulted in adsorption of 1a antigens from the medium such that 20% of the cells were 1a positive at a 1/4 dilution of the supernatant. A purer 1a antigen preparation was made from LPS stimulated spleen cell supernatant using a monoclonal anti-1a antibody column, and this was checked by immune blotting and SDS-FAGE. This preparation also adsorbs onto cells and is shown to influence mixed lymphocyte reactions. When BIO or Balb/b (H-2b) cells were used as stimulators with BIO.BR or Balb/k (H-2k) as responders, the inclusion of 1a antigens (ie. syngeneic to responders) significantly reduces the response. With BIO.BR as stimulators and BIO as responders, addition of la causes a significant increase in response although using Balb/k as stimulators and Balb/b as responders no difference is seen between presence and absence of 1a. The possibility of soluble la antigens modulating the immune response is discussed. When peripheral blood mononuclear cells (PBM) are treated with potassium superoxide solution (3x10.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: Adam Curtis
Keywords: Immunology
Date of Award: 1984
Depositing User: Enlighten Team
Unique ID: glathesis:1984-71772
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 17 May 2019 09:31
Last Modified: 17 May 2019 09:31
URI: http://theses.gla.ac.uk/id/eprint/71772

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