Effects of Bordetella pertussis on neutrophil leukocytes

Craig, Frank Furlong (1987) Effects of Bordetella pertussis on neutrophil leukocytes. PhD thesis, University of Glasgow.

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Abstract

This thesis describes and discusses the effects that various components of Bordetella pertussis produce on neutrophil leukocytes; particularly those of the rabbit. The work was done with crude fractions and purified components of the bacterium and also whole-cells of mutant strains and of antigenically-modulated variants of B. pertussis. The neutrophil functions which were investigated in detail were Cl) adhesiveness, as assayed by aggregation. C2D bactericidal response, as assayed by luminol-enhanced chemiluminescence, to a pro-inflammatory mediator (N'-formyl-L-methionyl-L-leucyl-L-phenylalanine; FMLP) or to intact X-mode B.pertussis Tohama. The filamentous haemagglutinin (FHA) of B. pertussis had at least four noteworthy effects on the function of rabbit peritoneal neutrophils: it enhanced neutrophil aggregation; it directly induced neutrophil chemiluminescence; it enhanced the neutrophil response to FMLP; and it induced a faster rate of chemiluminescence production to intact B. pertussis. There were species differences in neutrophils and FHA-enhancement of aggregation with rabbit neutrophils was not found with human or pig cells. Lipopolysaccharide endotoxin CLPS) of B. pertussis, unlike FHA, had no effect on the aggregation of rabbit peritoneal neutrophils. However, it resembled FHA in inducing neutrophil chemiluminescence directly and in enhancing neutrophil response to FMLF. LPS from Salmonella minnesota behaved similarly in each of the above. B. pertussis LPS also induced a faster rate of neutrophil chemiluminescence to intact B. pertussis. Pertussis toxin CPTD enhanced aggregation of rabbit peritoneal and peripheral neutrophils and human peripheral neutrophils. It inhibited neutrophil chemiluminescent response to FMLP although only after a lag period of 30-40 min. PT overrode the enhancing effects of FHA and LPS on neutrophil response to FMLP. The latter observation was applied to monitoring the toxoiding of an experimental acellular pertussis vaccine, consisting predominantly of PT and FHA. PT slightly suppressed the neutrophil chemiluminescent response to intact B. pertussis. With intact B. pertussis there was a positive correlation between hydrophobicity and the induction of neutrophil chemiluminescence. The work supports the view that a significant feature of infection with B. pertussis is the ability of the pathogen to impair the functional effectiveness of host phagocytes.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Advisers: Roger Parton; John M Lackie; John H Freer
Keywords: Pathology
Date of Award: 1987
Depositing User: Enlighten Team
Unique ID: glathesis:1987-72289
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 24 May 2019 15:12
Last Modified: 24 May 2019 15:12
URI: http://theses.gla.ac.uk/id/eprint/72289

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