Regulation of mannan synthesis in Saccharomyces cerevisiae

Harrington, Charles R (1980) Regulation of mannan synthesis in Saccharomyces cerevisiae. PhD thesis, University of Glasgow.

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Although a great deal is known about the chemical structure and enzymatic mechanisms involved in the assembly of yeast mannoproteins, little is known about the regulation of mannan biosynthesis. The aim of this study, therefore, was to investigate the control mechanisms involved. Two approaches were used. Firstly, the potential role of low molecular-weight effectors of mannosyltransferases was investigated. Secondly, the possible existence of an activation-inactivation mechanism of control, analogous to that which operates during chitin synthesis was explored. Mannosyltransferase activity in sphaeroplast lysates and washed membranes prepared from Saccharomyces cerevisiae X2180 was measured by following the incorporation of [14C]cjmannose from GDP-[14C]nBnnose into material precipitable with cold 0.3M perchloric acid. Mannosyl- transferase activity was optimal at low enzyme protein concentrations (l mg/ml), 50

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: L J Douglas
Keywords: Biochemistry
Date of Award: 1980
Depositing User: Enlighten Team
Unique ID: glathesis:1980-72319
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 24 May 2019 15:12
Last Modified: 24 May 2019 15:12

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