Modulation of spontaneous Ca2+ release from the sarcoplasmic reticulum by FKBP12.6 in rabbit ventricular cardiomyocytes

Loughrey, Christopher Michael (2003) Modulation of spontaneous Ca2+ release from the sarcoplasmic reticulum by FKBP12.6 in rabbit ventricular cardiomyocytes. PhD thesis, University of Glasgow.

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Abstract

Spontaneous sarcoplasmic reticulum (SR) Ca2+-release and propagated intracellular Ca2+ waves are a consequence of cellular Ca2+ overload in cardiomyocytes. We examined the relationship between the average intracellular [Ca2+] and Ca2+ wave characteristics. Amplitude, time course and propagation velocity of waves were measured using line-scan confocal imaging of beta-escin permeabilised cardiomyocytes perfused with 10muM Fluo-3 or Fluo-5F. Spontaneous Ca2+ waves were evident at cellular [Ca2+] >200nM. Peak [Ca2+] during a wave was 2-2.2)iM: minimum [Ca2+] between waves was 120-160nM; wave frequency was -0.1 Hz. Raising mean cellular [Ca2+] caused increases in all three parameters, particularly Ca2+ wave frequency. Increased rate of SR Ca.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: Godfrey Smith
Keywords: Physiology
Date of Award: 2003
Depositing User: Enlighten Team
Unique ID: glathesis:2003-72497
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 11 Jun 2019 11:06
Last Modified: 11 Jun 2019 11:06
URI: http://theses.gla.ac.uk/id/eprint/72497

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