Studies on the structure and replication of canine distemper virus

Armitage, Aileen Margaret Thomson (1976) Studies on the structure and replication of canine distemper virus. PhD thesis, University of Glasgow.

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Abstract

The principal aim of the investigation was to gain detailed information on the structure and replication of canine distemper virus. The first part of the thesis was intended to provide information on the growth of the virus in tissue culture to that a method could be established for the production of the large quantities of virus required for structural and biochemical studies. The growth of the virus in secondary dog kidney cultures, primary alveolar macrophage cultures, a canine embryonic cell strain, and the G-H continuous dog kidney cell line was compared. Secondary dog kidney cultures were the most susceptible to the virus but the highest yields were obtained from the GPI cells. By comparing different methods of infecting and maintaining the cells, it was found that large quantities of virus could best be obtained by infecting GH cells in monolayer and collecting the medium two or three times per day, once a clearly recognisable cytopathic effect was present. At this stage, virus accumulated rapidly in the medium, maximum titres being reached within a few hours of a change of medium; its short half-life at 37

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: H JC Cornwell
Keywords: Veterinary science, Virology
Date of Award: 1976
Depositing User: Enlighten Team
Unique ID: glathesis:1976-73124
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jun 2019 08:56
Last Modified: 14 Jun 2019 08:56
URI: http://theses.gla.ac.uk/id/eprint/73124

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