Studies on fermentative mechanisms in Zymosarcina ventriculi

Bauchop, T (1960) Studies on fermentative mechanisms in Zymosarcina ventriculi. PhD thesis, University of Glasgow.

Full text available as:
[img]
Preview
PDF
Download (23MB) | Preview

Abstract

1. The enzymes hexokinase, aldolase and triosephesphate dehydrogenase have been demonstrated in crude extracts of Z. ventriculi. 2. Pyruvate formation from 3-phosphoglyceric acid has also been obtained with crude extracts. 3. The fermentation of glucose by whole cells of Z. Ventriculi has been shown to be inhibited by iodoacetate but not by fluoride. 4. With toluene-treated cells of Z. ventriculi pyruvate is fermented mainly to CO2 and acetaldehyde, with the formation of smaller amounts of acetein, lactate and acetate. 5. The evolution of CO2 from pyruvate by toluene-treated cells has been shown to be sensitive to the inhibitors of a yeast-type carboxylase. 6. At pH 4.9 whole cells of Z. ventriculi ferment pyruvate quantitatively to equimolar amounts of acetate, H2 and CO2. 7. Formic hydrogenlyase, formic dehydrogenase and hydrogenase have been demonstrated with washed-cell suspensions of Z. ventriculi. 8. The formic hydrogenlyase system has been shown to be sensitive to the inhibitors of the formic hydrogenlyase system in E. coli. 9. On the basis of these findings, a scheme for the fermentation of glucose by Z. ventriculi has been propsed which accounts for the fermentation balances obtained by earlier workers. 10. The results indicate that Z. ventriculi is unique in being the only organism which possesses both a yeast-type carboxylase and a elastic reaction for the metabolism of pyruvate.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: E A Dawes
Keywords: Biochemistry
Date of Award: 1960
Depositing User: Enlighten Team
Unique ID: glathesis:1960-73149
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jun 2019 08:56
Last Modified: 14 Jun 2019 08:56
URI: http://theses.gla.ac.uk/id/eprint/73149

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year