Exploring complex chemical systems: insights from mass spectrometry, electrochemistry and continuous cell culture

Robbins, Philip James (2016) Exploring complex chemical systems: insights from mass spectrometry, electrochemistry and continuous cell culture. PhD thesis, University of Glasgow.

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Abstract

The work presented herein covers a broad range of research topics and so, in the
The work presented herein covers a broad range of research topics and so, in the interest of clarity, has been presented in a portfolio format. Accordingly, each chapter consists of its own introductory material prior to presentation of the key results garnered, this is then proceeded by a short discussion on their significance.
In the first chapter, a methodology to facilitate the resolution and qualitative assessment of very large inorganic polyoxometalates was designed and implemented employing ion-mobility mass spectrometry. Furthermore, the potential of this technique for ‘mapping’ the conformational space occupied by this class of materials was demonstrated. These claims are then substantiated by the development of a tuneable, polyoxometalate-based calibration protocol that provided the necessary platform for quantitative assessments of similarly large, but unknown, polyoxometalate species. In addition, whilst addressing a major limitation of travelling wave ion mobility, this result also highlighted the potential of this technique for solution-phase cluster discovery. The second chapter reports on the application of a biophotovoltaic electrochemical cell for characterising the electrogenic activity inherent to a number of mutant Synechocystis strains. The intention was to determine the key components in the photosynthetic electron transport chain responsible for extracellular electron transfer. This would help to address the significant lack of mechanistic understanding in this field. Finally, in the third chapter, the design and fabrication of a low-cost, highly modular, continuous cell culture system is presented. To demonstrate the advantages and suitability of this platform for experimental evolution investigations, an exploration into the photophysiological response to gradual iron limitation, in both the ancestral wild type and a randomly generated mutant library population, was undertaken. Furthermore, coupling random mutagenesis to continuous culture in this way is shown to constitute a novel source of genetic variation that is open to further investigation.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: ion mobility mass spectrometry, polyoxometalates, biophotovoltaics, cyanobacteria, continuous cell culture, experimental evolution, iron limitation photophysiology.
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Colleges/Schools: College of Science and Engineering > School of Chemistry
Supervisor's Name: Cronin, Professor Leroy
Date of Award: 2016
Embargo Date: 16 June 2019
Depositing User: Mr Philip Robbins
Unique ID: glathesis:2016-7321
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 17 May 2016 12:37
Last Modified: 20 May 2019 12:35
URI: http://theses.gla.ac.uk/id/eprint/7321

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