Exploring complex chemical systems: insights from mass spectrometry, electrochemistry and continuous cell culture

Robbins, Philip James (2016) Exploring complex chemical systems: insights from mass spectrometry, electrochemistry and continuous cell culture. PhD thesis, University of Glasgow.

Due to Embargo and/or Third Party Copyright restrictions, this thesis is not available in this service.


The work presented herein covers a broad range of research topics and so, in the
interest of clarity, has been presented in a portfolio format. Accordingly, each
chapter consists of its own introductory material prior to presentation of the key
results garnered, this is then proceeded by a short discussion on their
In the first chapter, a methodology to facilitate the resolution and qualitative
assessment of very large inorganic polyoxometalates was designed and
implemented employing ion-mobility mass spectrometry. Furthermore, the
potential of this technique for ‘mapping’ the conformational space occupied by
this class of materials was demonstrated. These claims are then substantiated by
the development of a tuneable, polyoxometalate-based calibration protocol that
provided the necessary platform for quantitative assessments of similarly large,
but unknown, polyoxometalate species. In addition, whilst addressing a major
limitation of travelling wave ion mobility, this result also highlighted the
potential of this technique for solution-phase cluster discovery.
The second chapter reports on the application of a biophotovoltaic electrochemical cell for characterising the electrogenic activity inherent to a
number of mutant Synechocystis strains. The intention was to determine the key
components in the photosynthetic electron transport chain responsible for
extracellular electron transfer. This would help to address the significant lack of
mechanistic understanding in this field.
Finally, in the third chapter, the design and fabrication of a low-cost, highly
modular, continuous cell culture system is presented. To demonstrate the
advantages and suitability of this platform for experimental evolution
investigations, an exploration into the photophysiological response to gradual
iron limitation, in both the ancestral wild type and a randomly generated mutant
library population, was undertaken. Furthermore, coupling random mutagenesis
to continuous culture in this way is shown to constitute a novel source of genetic
variation that is open to further investigation.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: ion mobility mass spectrometry, polyoxometalates, biophotovoltaics, cyanobacteria, continuous cell culture, experimental evolution, iron limitation photophysiology
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Colleges/Schools: College of Science and Engineering > School of Chemistry
Supervisor's Name: Cronin, Professor Leroy
Date of Award: 2016
Embargo Date: 16 May 2019
Depositing User: Mr Philip Robbins
Unique ID: glathesis:2016-7321
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 17 May 2016 12:37
Last Modified: 26 May 2016 10:10
URI: http://theses.gla.ac.uk/id/eprint/7321

Actions (login required)

View Item View Item