The effect of the viscosity of the medium on the reactions of cells to topography

Khorshid, Faten (2001) The effect of the viscosity of the medium on the reactions of cells to topography. PhD thesis, University of Glasgow.

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Abstract

The viscosity of the medium in which cells grow may alter their cellular reaction to topography and this in turn may affect cell growth, morphology and movement. In this study, cells have been grown in medium with high viscosity on plane and grooved topography in order to investigate the effect of the viscosity of the medium on the reaction of cells to topography. The viscosity was changed by adding viscous macromolecules, such as dextran, ficoll, polyvinylpyrrolidone, Methylcellulose and Carboxymethylcellulose to the normal medium (ECT or Ham's F10). Due to other solution properties that might be altered by the change of medium viscosity, such as osmolarity or chemical interactions, cells were examined in the presence of a variety of concentrations of macromolecules, which differed in their size, capacity to elevate fluid viscosity, and in their chemical nature. The medium viscosity was determined by measuring its flow time, relative to water, using an Ostwald viscometer. All measurements were performed at 37

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: Adam Curtis
Keywords: Cellular biology
Date of Award: 2001
Depositing User: Enlighten Team
Unique ID: glathesis:2001-73428
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jun 2019 08:56
Last Modified: 14 Jun 2019 08:56
URI: http://theses.gla.ac.uk/id/eprint/73428

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