An investigation into the growth and antigenic properties of adenoviruses detected by electron microscopy in the stools of children in Glasgow

Kidd, Alistair H (1980) An investigation into the growth and antigenic properties of adenoviruses detected by electron microscopy in the stools of children in Glasgow. PhD thesis, University of Glasgow.

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Adenovirus particles which cannot be cultured have been found in the stools of children by various workers. To determine the extent of this problem, stools from children in Glasgow, sent to the Regional Virus Laboratory for electron microscopic examination over a 2-year period (1976-197.) and found to contain adenovirus particles, were put in culture. One third of 148 specimens containing adenovirus (from 28 of 69 children) failed to cause cytopathic effects in primary human amnion and secondary human embryo kidney (HEK) cell cultures. Variations in culture method such as rolling tubes or adsorption periods did not improve the adenovirus isolation frequency. There was no common factor apparent, concerning the patients or their stools, which night explain the non-growth of adenoviruses. Attempts to 'reactivate' non-growing adenoviruses by culture attempts in human cells in the presence of either adeno associated virus (AAV), human adenovirus (type 8) or a canine adenovirus (ICHV) were unsuccessful. AAV type 1 did not multiply in the presence of non-growing adenovirus particles. An immunofluorescence test using group-specific guinea pig antiserum revealed that with some stool extracts a small number of KB cells become infected and produce adenovirus structural antigens. Fluorescence was limited to single cells, indicating that, at 3 days after inoculation, spread of any new infectious virus to adjacent cells did not occur. Attempts to detect an inhibitory substance to adenovirus replication, which might explain the lack of cytopathic effects, were unsuccessful. Bovine cells, canine cells and various continuous human cell lines were tried for the detection of non-growing adenoviruses, and some success was achieved using Intestine 407 and Chang Conjunctiva cells maintained in Leibovitz' L15 medium. Two agents from different children caused cytopathic effects over 8 passages in Chang cells. Apart from these agents, the extent of the cytopathic effects declined in most cases with serial passage, but this decline was not so rapid if the cultures were incubated at 33

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: C R Madeley
Keywords: Virology
Date of Award: 1980
Depositing User: Enlighten Team
Unique ID: glathesis:1980-74088
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 23 Sep 2019 15:33
Last Modified: 23 Sep 2019 15:33

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