Actions of Oxygen-Derived Free Radicals and Reactive Oxygen Species on the Organelles of Force Production and Calcium Storage in Cardiac Muscle

MacFarlane, Niall Gordon (1993) Actions of Oxygen-Derived Free Radicals and Reactive Oxygen Species on the Organelles of Force Production and Calcium Storage in Cardiac Muscle. PhD thesis, University of Glasgow.

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Abstract

The work which comprises this thesis concerns the effects of oxygen-derived free radicals and reactive oxygen species on the intracellular organelles of force production and calcium storage in cardiac muscle. In addition, the possibility of endogenous histidine-containing compounds having antioxidant activity has been investigated. Preparations treated with Triton-X100 retain only traces of membrane structure while the contractile proteins remain functional. This form of treatment is ideal for investigating alterations to myofilament calcium sensitivity and maximum calcium-activated force production. Saponin-treated trabeculae retain the intracellular membranes associated with the sarcoplasmic reticulum and the mitochondria, while the sarcolemma is rendered permeable to the experimental solutions. This form of treatment is ideal for investigating alterations to sarcoplasmic reticulum and mitochondrial function.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: Dave Miller
Keywords: Physiology
Date of Award: 1993
Depositing User: Enlighten Team
Unique ID: glathesis:1993-74444
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 27 Sep 2019 18:20
Last Modified: 27 Sep 2019 18:20
URI: https://theses.gla.ac.uk/id/eprint/74444

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