Studies of a Rat Liver Ribonuclease Inhibitor Protein Gene

McKerrell, Margot Julie (1989) Studies of a Rat Liver Ribonuclease Inhibitor Protein Gene. PhD thesis, University of Glasgow.

Abstract

The cytoplasmic ribonuclease inhibitor-ribonuclease system has been detected in a wide variety of tissues and species and is thought to play an essential role in the regulation of RNA metabolism and hence protein synthesis. This hypothesis is based on the observation that tissues with high levels of RNA and protein synthesis are associated with correspondingly high levels of free inhibitor and vice versa. However, the precise nature of this regulatory function is unknown. Therefore, the aim of this project was to isolate cDNA clones of ribonuclease inhibitor from a rat liver library in the expression vector, lgt11 using polyclonal antiserum, to characterise the resultant clones and to use the clones to determine the extent to which ribonuclease inhibitor activities are controlled at the level of gene expression.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Biochemistry
• Date of Award: 1989
• Depositing User: Enlighten Team
• Unique ID: glathesis:1989-76938
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:27
• Last Modified: 14 Jan 2020 09:27
• URI: https://theses.gla.ac.uk/id/eprint/76938