Molecular Biological Studies of the Induction of Flowering

Ballard, Michelle Joy (1990) Molecular Biological Studies of the Induction of Flowering. PhD thesis, University of Glasgow.

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Abstract

Potential changes in gene expression which occur in the leaves during photoperiodic floral induction were investigated in a variety of plant species including Nicotiana plumbaginifolia (day neutral plant), Amaranthus caudatus (short day plant), Silene coeli-rosa (long day plant), and Anagallis arvensis (long day plant) which exhibit a range of photoperiodic responses. The approach taken was to employ techniques which would demonstrate differential gene expression at the level of mRNA or protein including in vitro translation of leaf mRNA and analysis of products using one- or two-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)1analysis of leaf total protein using one- or two-dimensional SDS-PAGE, differential immunoadsorbtion techniques for the identification of 'induced' specific proteins, and differential screening of an 'induced' cDNA library.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Botany, Plant sciences
Date of Award: 1990
Depositing User: Enlighten Team
Unique ID: glathesis:1990-76970
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jan 2020 11:53
Last Modified: 14 Jan 2020 11:53
URI: http://theses.gla.ac.uk/id/eprint/76970

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