Transposition of Tn7

Morrell, Elizabeth (1990) Transposition of Tn7. PhD thesis, University of Glasgow.

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Abstract

One of the transposition proteins of Tn7, TnsB, was overexpressed, and purified substantially. The protein has a subunit molecular weight of around 85 kDA, which is consistent with prediction from the DNA sequence, and appears to exist as a monomer in solution. The identity of the polypeptide was confirmed by N-terminal sequencing, which also demonstrated that the site of translational initiation is the first AUG in the reading frame, and that there is no N-terminal processing of the protein.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Genetics, Molecular biology
Date of Award: 1990
Depositing User: Enlighten Team
Unique ID: glathesis:1990-77006
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jan 2020 09:24
Last Modified: 14 Jan 2020 09:24
URI: http://theses.gla.ac.uk/id/eprint/77006

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