Development of a Cloning Strategy to Enable the Isolation of Novel Membrane Proteins

Hundal, Arvind M (1991) Development of a Cloning Strategy to Enable the Isolation of Novel Membrane Proteins. PhD thesis, University of Glasgow.

Abstract

The aim of the project was to develop a cloning strategy for the isolation of novel membrane proteins. The scheme evolved from the surface antigen screening system developed by Seed and Aruffo (1987). The system was adapted to enable the cloning of cDNAs coding for membrane proteins to which no antibodies exist. This was achieved by taking a previously cloned membrane protein gene (CD2), deleting its transmembrane (Tm) and cytosolic regions, and replacing them with the polylinker and stuffer sequences of CDM8. cDNAs were then sub-cloned into this expression vector to generate a fusion library. which was then screened for surface expression of the CD2 protein.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Genetics, Molecular biology
• Date of Award: 1991
• Depositing User: Enlighten Team
• Unique ID: glathesis:1991-77070
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77070