Mitogenic Signalling by the Endothelin Receptor in Rat-1 Fibroblasts

Mac Nulty, Elisabeth E (1992) Mitogenic Signalling by the Endothelin Receptor in Rat-1 Fibroblasts. PhD thesis, University of Glasgow.

Abstract

Endothelin-1 (ET-1) was found to be a complete mitogen in Rat-1 fibroblasts and therefore the signalling pathways which might mediate its action were investigated. ET-1 stimulated both sustained phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylcholine (PtdCho) hydrolysis. The rank order of potency for both pathways stimulated by a range of ET isopeptides was the same for both responses (ET-1 ~ ET-2 > ET-3) suggesting that the same receptor controls both signalling pathways. PtdCho hydrolysis occurred kinetically downstream of Ptdlns(4,5)p2 hydrolysis by a phospholipase D-catalysed mechanism apparently involving both protein kinase C-dependent and -independent means of activation.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Biochemistry
• Date of Award: 1992
• Depositing User: Enlighten Team
• Unique ID: glathesis:1992-77311
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77311