Laboratory and Experimental Studies of Prosthesis Related Oral Candidiasis

Jennings, Kevin John (1988) Laboratory and Experimental Studies of Prosthesis Related Oral Candidiasis. MSc(R) thesis, University of Glasgow.

Abstract

The nature of the histological appearance of clinically inflamed epithelium found under dentures in the condition of denture-induced stomatitis has been recorded in qualitative terms in the literature over many years. Information of a quantitative nature relating to changes in palatal epithelium in the condition is scarce. It was the aim of this work to make observations based on quantitative data describing the epithelial changes in denture-induced stomatitis. In the first part of the project, samples of palatal mucosa were collected from patients exhibiting the signs of denture-induced stomatitis. Quantitative stereological techniques were used in the analysis of tissue. Changes occurring were assessed by comparison of the findings with results from healthy oral epithelium similarly analysed by other workers. The importance of haematological and microbiological factors in the pathogensis of denture-induced stomatitis was examined. There was found to be a varied histological presentation within this single clinical entity. There also appeared to be a link between the haematological and microbiological findings. Further study of the cellular changes induced in the condition was continued by investigations using an animal model. The Wistar rat was the animal chosen for the model and further investigation of palatal epithelium within the animal model was in three parts. In the initial animal study Candida albicans was inoculated under intra-oral acrylic appliances, and epithelial changes occurring were analysed. Differences between experimental and control animals were found, but problems of quantification of the epithelium under consideration were experienced. Problems arose due to the presence of undulations, or rugae. covering all of the palatal epithelium of the Wistar rat. The second part of the animal study involved analysis of the histological structure of normal Wistar rat palatal epithelium and consideration of how such tissue could best be sampled to allow quantitative assessment. Tissue sections suitable for analysis on a quantitative basis were produced. The animal study concluded with a more extensive experimental investigation involving the Wistar rat animal model. Epithelial changes induced under experimental conditions were measured. Experimental groups consisted of animals wearing acrylic appliances which were of two differing designs. In some of the animals. Candida albicans was innoculated underneath appliances, whilst in the others, appliances were worn without additional experimental procedure. Tissue was sampled in a manner to allow quantitative methods of analysis. It was observed that changes were induced in the experimental animals and that these were primarily related to the presence of an acrylic appliance rather than to the inoculation of Candida albicans. The location of the tissue analysed was found to play an important part in determining the nature of the tissue changes induced. Histopathological change specifically related to the presence of Candida albicans were rare and appeared to occur only where ulceration of the epithelium was induced by the intra-oral appliance.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Keywords: Medicine
• Date of Award: 1988
• Depositing User: Enlighten Team
• Unique ID: glathesis:1988-77661
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77661