Analysis of the Transcripts of Bovine Papillomavirus Type 4

Stamps, Alasdair C (1987) Analysis of the Transcripts of Bovine Papillomavirus Type 4. PhD thesis, University of Glasgow.

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Abstract

The transcripts of bovine papillomavirus type 4 (BPV4) were analysed through cDNA cloning and sequencing. Two cDNA's of particular interest were analysed. cDNA 7E11 was found to represent the 1 kb major transcript of BPV4. Primer extension and S1 nuclease protection mapping showed that this transcript had a multiple initiation site within the E6 orf region, with the major start site at nt870. cDNA and RNA sequencing showed that the transcript was spliced from nt1016 to nt3376. The polyadenylated 3' terminus was at nt4034, defining the early polyadenylation site of BPV4. Sequencing of cDNA's and RNA revealed alterations to the published genomic sequence (Patel et al., 1987) which changed the genomic organisation of BPV4. cDNA 7E11 was shown to encode a fusion orf in which the E4 orf was linked to an upstream ATG codon. Thus, as in other papillomaviruses, the major transcript of BPV4 encodes one of the E4 proteins (Doorbar et al., 1986). Orf homology analysis revealed that the BPV4 E4 orf is most like those of the carcinogenic HPV's 16, 18, 33 and 8. Since the E4 proteins are thought to be involved in the subversion of normal keratinocyte differentiation, this may be a significant finding. In addition, rare transcripts represented by cDNA Q were mapped by RNA sequencing and S1 nuclease protection. A 1kb transcript, which may be spliced, extends 5' to nt2834 and minor 5' termini at nt3063, nt3093 and nt3151 may have represented transcripts encoding the putative E2 repressor of BPV4.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Virology
Date of Award: 1987
Depositing User: Enlighten Team
Unique ID: glathesis:1987-77665
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jan 2020 11:53
Last Modified: 14 Jan 2020 11:53
URI: http://theses.gla.ac.uk/id/eprint/77665

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