Thermodynamics of Biomolecular Recognition

McAuley-Hecht, Katherine E (1993) Thermodynamics of Biomolecular Recognition. PhD thesis, University of Glasgow.

Abstract

Thermodynamic aspects of molecular recognition have been studied using yeast phosphoglycerate kinase (PGK) and two antibiotics, ristocetin and vancomycin, as model systems. The binding of 3 natural substrates, ADP, ATP and 3-phosphoglycerate to wild-type and two mutant forms of PGK was examined using kinetic techniques and isothermal titration microcalorimetry. The latter technique was also used to investigate the binding of various ligand molecules to PGK and to study the formation of the ternary complex- PGK.3-PG.ADP. The thermodynamic parameters (Ka, DeltaH

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: Alan Cooper
• Keywords: Biophysics, Molecular biology, Thermodynamics
• Date of Award: 1993
• Depositing User: Enlighten Team
• Unique ID: glathesis:1993-75338
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 19 Nov 2019 20:49
• Last Modified: 19 Nov 2019 20:49
• URI: https://theses.gla.ac.uk/id/eprint/75338