An Investigation of the Cellular Stress Response in Cells Infected With Herpes Simplex Virus

Notarianni, Elena (1986) An Investigation of the Cellular Stress Response in Cells Infected With Herpes Simplex Virus. PhD thesis, University of Glasgow.

Abstract

This study entailed an investigation of the cellular stress response in secondary chick embryo fibroblasts (CEF) during infection by HSV. It was established that infections at a+ NPT with temperature-sensitive mutants of HSV-1 which are defective in immediate-early viral polypeptide Vmw IE 175 (i.e. tsD, tsK and tsT) cause the stress response to be induced, as manifest by a marked stimulation of synthesis of stress proteins. Induction by tsK was shown to be dependent upon the synthesis of immediate-early viral polypeptides. Infections with other mutants of HSV-1 (tsl201, tsB, tsE, tsG and MDK/2) at a NPT and, to a lesser extent, the revertant of tSK, ts+K, or with wt HSV-1 or wt HSV-2, all of which are non-defective in Vmw IE 175 or the HSV-2 equivalent, Vmw IE 182, cause synthesis of stress proteins to be increased. To account for these observations a hypothesis was advanced, that cells are subjected to stress during infection with wt or mutant HSV, owing to the expresssion of viral functions, and that induction of the stress response is subsequently inhibited depending upon the characteristics of the infecting virus: inhibition is most effective in cells infected with wt viruses, and least effective in cells infected at a NPT with temperature-sensitive mutants of HSV-1 defective in Vmw IE 175.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Virology
• Date of Award: 1986
• Depositing User: Enlighten Team
• Unique ID: glathesis:1986-77468
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77468