The milk proteome and the acute phase response during bovine mastitis

Hogarth, Caroline J. (2004) The milk proteome and the acute phase response during bovine mastitis. PhD thesis, University of Glasgow.

Full text available as:
[thumbnail of 2004hogarthphd.pdf] PDF
Download (24MB)
Printed Thesis Information:


The principal aims of the work presented in this thesis were to further investigate the
pathophysiology of the acute phase proteins in bovine mastitis and to document the changes in the
protein composition of milk during mastitis using modem proteomic methods.
Mastitis is one of the most important diseases affecting cattle worldwide. It has adverse effects on
the economics of milk production through reducing the quality and quantity of milk. Acute phase
proteins (APPs) are valuable markers of inflammatory lesions and are widely used in the veterinary
field for disease diagnosis. This study further investigated the presence of APPs, haptoglobin (Hp) 1 I
and mammary- associated serum amyloid A3 (M-SAA3) in the milk of cows with mastitis and
evaluated the possibility of using these proteins to detect mastitis.
An Enzyme linked immunosorbent assay (ELISA) was developed and validated for the
measurement of milk Hp. A commercially available ELISA was used for the detection ofM-SAA3
in milk. Both Hp and M-SAA3 showed similar kinetics to somatic cell counts and can be
considered as sensitive and reliable markers of mastitis.
Advanced proteomic methods were developed and used to further characterise the changes taking
place in the proteins expressed in normal and mastitic milk. The methodology for analysis of the
bovine milk proteome was successfully established. This part of the work concluded that the
patterns of protein expression of clinically mastitic milk showed clear differences from that of
normal milk. Further analysis of milk samples from a mild subclinical model of Staphylococcus
aureus mastitis showed that alterations to the milk p:oteome were only minimal. However this
study was useful in identifying areas worthy of future research.
Recent publications on APP research speculate upon the local production ofM-SAA3 in the bovine mammary gland. The final objective of this study was therefore to investigate the expression of MSAA3 in bovine mammary tissue. Our studies confirm the local production of M-SAA3 in the
bovine mammary gland and its up-regulation during bovine mastitis. This finding may provide
further information on a possible role for this protein.
Overall the findings detailed in this thesis indicate that APPs in milk are valuable markers of
mastitis and additionally that the ease of collection of milk by non-invasive methods suggest that
milk has potential for the discovery of disease biomarkers.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Subjects: S Agriculture > SF Animal culture > SF600 Veterinary Medicine
Colleges/Schools: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Supervisor's Name: Eckersall, Prof. David, Nolan, Prof. Andrea and Fitzpatrick, Prof. Julie
Date of Award: 2004
Depositing User: Mrs Marie Cairney
Unique ID: glathesis:2004-4931
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 06 Feb 2014 13:55
Last Modified: 06 Feb 2014 14:01

Actions (login required)

View Item View Item


Downloads per month over past year