Pol5+, a potential link between cell cycle and cell growth in fission yeast

Nadeem, Farzana Khaliq (2005) Pol5+, a potential link between cell cycle and cell growth in fission yeast. PhD thesis, University of Glasgow.

Abstract

SpCdc10p is the major component of the cell cycle regulated transcription factor complex DSC1 (DNA synthesis control 1) in fission yeast. DSC1 regulates the periodic transcription of a set of genes at the G1-S transition, which is required to pass the mitotic G1 checkpoint termed 'START' and also for S phase progression. The promoters of DSC1 regulated genes contain a common cw-acting element called MCB (Mlu1 cell cycle box) that binds to DSC1 to confer periodic gene expression. Some of the genes required for S phase include cdc22+ (large subunit of ribonuleotide reductase), cdc18+ and cdt1+ (DNA replication licensing factors) and cig2+ (the major S phase cyclin). cig2+ interacts cyclically with the cyclin-dependant kinase, SpCdc2p, to pass START and enter mitotic division. Both budding yeast and mammalian cells contain homologous gene expression systems and are controlled by DSC1-like activities called SBF/MBF and E2F in yeast and humans, respectively. The aim of this study was to isolate novel proteins that interact with SpCdc10p, with particular focus on the 61 amino acid C-terminal region believed to be important for its regulation. Two-hybrid analysis and co-immunoprecipitation assays revealed a novel protein called SpPo1Sp. The two-hybrid experiment suggested a potentially strong interaction between SpPo1Sp and the C-terminal of SpCdc10p and the co- immunoprecipitation assays confirmed that the interaction was direct between these two proteins. Recently, ScPo1Vp, the budding yeast homologue of SpPo1Sp was identified and shown to be involved in rRNA synthesis, with a less important role as a DNA polymerase. Relatively little is known about SpPo1Sp function and it was proposed that it had the same role as ScPo1Vp due to its amino acid sequence similarities. In this thesis pol5.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Cellular biology.
• Colleges/Schools: College of Medical Veterinary and Life Sciences
• Supervisor's Name: McInerny, Dr. Chris
• Date of Award: 2005
• Depositing User: Enlighten Team
• Unique ID: glathesis:2005-71455
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 10 May 2019 14:37
• Last Modified: 28 Jul 2021 14:14
• URI: https://theses.gla.ac.uk/id/eprint/71455