Ovine bronchopulmonary globule leucocytes

Mahmoud, Ghyath Salih (1978) Ovine bronchopulmonary globule leucocytes. PhD thesis, University of Glasgow.

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The main objectives of this thesis were to demonstrate that globule leucocytes can be found in the bronchopulmonary system of sheep and to study the characteristic features of globule leucocytes and mast cells in parasitic bronchopulmonary diseases of sheep. Subsequently a survey on the occurrence of these cells in the tracheobronchial tree of groups of sheep, kept in different management systems. These cells were found in the trachea, bronchi, bronchioles and bronchial glands of sheep which had parasitic pulmonary lesions. Quantitative studies of the number of globule leucocytes at standard sites in the bronchopulmonary system revealed a close association between these cells and parasitic infection of the respiratory tract, although considerable variation was found in the quantification of globule leucocytes between different lobes of each lung. A similar variation occurred between the standard sites of sampling within the same lobe. This study indicated that the lobes of lungs which harboured more parasitic lesions quantitatively had more globule leucocytes in their epithelial mucosae. Histochemical methods demonstrated the presence of sulphomucins in the globules of globule leucocytes and granules of mast cells. Both of these cell types were his to chemically related but differed from eosinophil leucocytes in lung tissue. Ultrastructurally, both globule leucocytes and mast cells of the lamina propria contained numerous intracytoplasmic granules, the maxtrixes of which were either completely electron dense and homogeneous or less dense with a fine granular ultrastructure. Globule leucocytes were most numerous in the surface epithelium and were not connected by desmosomes to the epithelial cells. The relationship between the globule leucocyte, mast cell and other cells in the respiratory tract of sheep during the course of a parasitic infection was briefly discussed with reference to recent reports on the presence of these cells in the intestinal wall of certain animals including sheep. Globule leucocytes were induced to develop in the tracheobronchial epithelium of lambs experimentally infected with Ascarls suum eggs, Dictyocaulus filaria and Haemonchus contortus larvae. Investigations showed that experimental infection with D. filaria in lambs produced an abundant increase in the number of bronchopulmonary globule leucocytes when compared with other types of experimental infection. Another difference between these experimental infections was revealed in the time required for the appearance and disappearance of these cells in the tracheobronchial tree of sheep. Exposure of lambs to the dust of mouldy hay and experimental endobronchial inoculation of lambs with Aspergillus fumigatus spores failed to induce any globule leucocytes in their tracheobronchial tree. The main pathological lesions in all experimental cases were tracheitis, bronchitis and bronchiolitis with peribronchial cellular accumulations and an exudate in the lumen. The majority of the cases had pulmonary eosinophilia, while the remaining lungs had lymphocytic and plasma cell infiltration. The literature relevant to the study of globule leucocytes and mast cells in sheep was reviewed and discussed.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: H M Pirie
Keywords: Animal diseases
Date of Award: 1978
Depositing User: Enlighten Team
Unique ID: glathesis:1978-72432
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 24 May 2019 15:12
Last Modified: 24 May 2019 15:12
URI: http://theses.gla.ac.uk/id/eprint/72432

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