Indole-3-acetic acid in Phaseolus vulgaris L

McDougall, John (1978) Indole-3-acetic acid in Phaseolus vulgaris L. PhD thesis, University of Glasgow.

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An analytical method has been developed in order that the amount and distribution of IAA in shoot tissue of Phaseolus vulgaris could be determined. A variety of extraction and purification procedures were investigated to devise a reliable method for the purification of shoot tissue extracts. By combining those methods that were shown to lead to an overall reduction in sample contamination, a procedure was developed that was capable of yielding a sample of suitable purity for subsequent analysis by gas chromatography and mass spectrometry. This procedure involved an initial methanol extraction period followed by solvent partitioning prior to the purification of the extract on columns of DEAE-cellulose and PVP, as well as thin layer chromatography. For quantitative analysis of the total IAA in these purified samples an electron-capture GC system and a GC-MS procedure were tested on the basis of sensitivity and specificity. The GC-MS system using previously trimethylsilylated plant samples was found to be more suitable. In addition to this analytical procedure, a method involving radio-GLC was developed in order to gauge the recovery of radioactive IAA at the completion of the purification procedure. The most efficient methods were then combined into one experimental procedure and applied to the assay of IAA in shoot tissue extracts of P. vulgaris. The results of this analysis indicated that the Level of IAA was in the range of 1.7-3.8 mug Kg-1 fresh weight. The distribution of IAA in the foliar and stem tissue was subsequently gauged. In the foliar tissue samples, the apex was found to contain the greatest Level of IAA (7-62 mug kg-1) with older trifoliate leaves having the second largest Level (2-20 mug kg-1). The lowest Level of IAA was found in the primary leaf tissue (0.4 -3.5 g kg-1). For the stem tissue experiments no distinct gradient was apparent, but the intermodal region between the cotyledons and the primary leaves was found to have the least amount of IAA.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: J R Hillman
Keywords: Plant sciences
Date of Award: 1978
Depositing User: Enlighten Team
Unique ID: glathesis:1978-72436
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 24 May 2019 15:12
Last Modified: 24 May 2019 15:12

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