In vitro biosynthesis of 1,4-beta-galactan attached to a pectin-xyloglucan complex in peas

Abdel-Massih, Roula M (2001) In vitro biosynthesis of 1,4-beta-galactan attached to a pectin-xyloglucan complex in peas. PhD thesis, University of Glasgow.

Abstract

Cell-wall matrix polysaccharides are synthesised in the Golgi apparatus and then transported to the cell wall. We have investigated the properties of nascent pectin formed by the biosynthetic system in vitro. Particulate enzyme preparations were prepared from etiolated pea epicotyls (Baydoun et al, 2001) and used to assay for 1,4-beta-galactan synthase using UDP-[U-14C]galactose. Optimum conditions for 1,4-beta-galactan synthesis were determined. The enzyme products were characterised by selective enzymatic degradation and anion-exchange chromatography. Evidence was obtained for the formation of 1,4-beta-galactan chains attached to a pectic backbone containing both polygalacturonic acid and rhamnogalacturonan I. The results also indicated that part or all of this nascent pectin was present as a complex with xyloglucan. This complex may be similar to pectin-xyloglucan complexes found in the cell wall in rose suspension cells (Thompson and Fry, 2000) and cauliflower stem (Femenia et al., 1999).

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: Chris Brett
• Keywords: Plant sciences
• Colleges/Schools: College of Medical Veterinary and Life Sciences
• Supervisor's Name: Bett, Dr. Chris and Baydoun, Professor Elias
• Date of Award: 2001
• Depositing User: Enlighten Team
• Unique ID: glathesis:2001-72556
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 11 Jun 2019 11:06
• Last Modified: 10 Dec 2021 15:02
• URI: https://theses.gla.ac.uk/id/eprint/72556