Gene transfer in Bordtella pertussis

Al-Sallami, Saleh Fadhel Mohsin (1981) Gene transfer in Bordtella pertussis. PhD thesis, University of Glasgow.

Abstract

In order to have available a procedure for gene transfer in Bordetella pertussis, the transformation system described originally by Branefors (1964) was re-examined and further developed. Under her original conditions, few streptomycin-resistant transformants were obtained in the present investigation when DNA prepared from the 44122/7R streptomycin-resistant strain was incubated with competent 44-122/7 streptomycin-sensitive cells. In attempts to improve the transformation frequency, conditions for DNA isolation, preparation of competent cells and exposure of recipient cells to DNA were examined in detail, Best results were obtained when bacteria (10.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Advisers: Roger Parton; John Coote
• Keywords: Genetics
• Date of Award: 1981
• Depositing User: Enlighten Team
• Unique ID: glathesis:1981-72609
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 11 Jun 2019 11:06
• Last Modified: 11 Jun 2019 11:06
• URI: https://theses.gla.ac.uk/id/eprint/72609