Cell surface changes induced by vaccinia virus

Liddell, Kenneth G (1978) Cell surface changes induced by vaccinia virus. PhD thesis, University of Glasgow.

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Virus-specified antigens induced in the membranes of infected cells have been implicated as primary targets in the recognition and destruction of virus-infected cells by the immune system of mammalian hosts. Antigenic changes induced in the plasma membranes of vaccinia-infected HeLa cells 2 h post-infection were detected by immunofluorescence, immune haemadsorption, and to a lesser extent by complement-fixation and complement-mediated cytolysis. Cytopathic effects (cell rounding) were seen prior to viral DM replication and also when this was blocked. Other changes occurred at the cell surface later in infection when turkey erythrocytes and concanavalin A were bound by cells. There was no evidence for an overall increase in mucopolysaccharide synthesis in the form of sialic acid. Quantitative spectrophotometric assays for haemadsorption, immune haemadsorption and concanavalin A binding were developed and the effect of varying growth or reaction conditions on the expression of vaccinia haemagglutinin at the surface of infected cells was investigated. Vaccinia cell surface haemagglutinin (VHA), detected by the binding of turkey erythrocytes, was synthesised around 10 h post-infection, even under conditions of low multiplicity of infection, and was closely coincident with the appearance of infectious virus. Of seven different cell lines tested, haemadsorption was greatest in HeLa cells and least in L-929 cells. Other red cell species were bound to infected cells to a lesser degree than turkey cells. Haemadsorption occurred within the physiological and alkaline pH ranges and at temperatures between 20

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: T H Birkbeck
Keywords: Virology
Date of Award: 1978
Depositing User: Enlighten Team
Unique ID: glathesis:1978-73178
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jun 2019 08:56
Last Modified: 14 Jun 2019 08:56
URI: https://theses.gla.ac.uk/id/eprint/73178

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