Neutrophil leucocyte adhesion under flow conditions

Chaabane, Nedjma (1987) Neutrophil leucocyte adhesion under flow conditions. MSc(R) thesis, University of Glasgow.

Abstract

The adhesion of neutrophil leucocytes to overslips coated with plasma proteins has been studied under both stastic and flow conditions. Neutrophils adhered poorly to both fibronectin and cryoprecipitate-coated glass. The effect of the proteins tested on neutrophil adhesion was dose dependent, with a pronounced effect at 100 mug/ml of protein, which was then reduced at lower doses. Compared to clean glass (control), the effect of serum, HSA, fibronectin-depleted cryopreciptate, fibronectin-depleted plasma and Factor VIII was to reduce adhesion but by an insignificant amount, whereas, cryoprecipitate and in particular fibronectin dramatically inhibited adhesion. This suggests that plasma components in general are not effective in reducing leucocyte adhesion, although fibronectin-coated surfaces are very non-adhesive. The speed with which neutrophils responded to chemotactic factors in the medium was tested in the flow chamber assay using a Y-shaped manifold for perfusing cells and chemotactic factors through a chamber precoated with BSA immediately after mixing. Neutrophil response to FMLP was dose dependent. A high concentration of FMLP (10.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Keywords: Immunology.
• Colleges/Schools: College of Medical Veterinary and Life Sciences
• Supervisor's Name: Lackie, Dr. J.
• Date of Award: 1987
• Depositing User: Enlighten Team
• Unique ID: glathesis:1987-73264
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jun 2019 08:56
• Last Modified: 10 Jun 2021 15:25
• URI: https://theses.gla.ac.uk/id/eprint/73264