The isolation and characterisation of temperature-sensitive mutants of R. S. virus

Faulkner, Georgine Potter (1975) The isolation and characterisation of temperature-sensitive mutants of R. S. virus. PhD thesis, University of Glasgow.

Abstract

The RSN-2 strain of RS virus grown in BS-C-1 cells was used for the isolation and characterisation of ts mutants. RSN-2 strain growth was examined at different temperatures and the degree of cell-association of the virus calculated. The effect of DM inhibitors such as actinomycin D and mitomycin C as well as the effect of the concentration of phenylalanine, arginine and serum on RS virus was studied. Thin sections of RS virus infected cells were examined by electron microscopy. Infected cells were also examined under a scanning electron microscope. Thirty-five ts mutants of the RSN-2 strain were isolated from mutagenised virus and one ts mutant from non-mutagenised virus. A few ts mutants were ts at 37

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: C R Pringle
• Keywords: Virology
• Date of Award: 1975
• Depositing User: Enlighten Team
• Unique ID: glathesis:1975-74250
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 23 Sep 2019 15:33
• Last Modified: 23 Sep 2019 15:33
• URI: https://theses.gla.ac.uk/id/eprint/74250