The Construction and Analysis of Bispecific Antibodies

Smith, Carron (1993) The Construction and Analysis of Bispecific Antibodies. MSc(R) thesis, University of Glasgow.

Abstract

Three different cross-linking reagents were examined for their ability to link two antibodies together (anti-CD3 and anti- ovarian carcinoma) to produce functional 'bispecific' antibodies, -that is, antibodies capable of directing cytotoxicity towards ovarian cancer cell targets. The cross-linking of antibodies by chemical means proved to be a difficult task. The cross-linker SMPB, was found to be impractical for use on a routine basis, and resulted in a high loss of antibody due to the number of steps involved in the process. SPDP and O-PDM, on the other hand, did appear to produce 'bispecific' antibodies, which were the correct size by gel filtration chromatography and which also produced 'positive' fluorescence by flow cytometry. Success was also achieved in producing antibodies capable of performing some directed cytotoxicity. This investigation, however, also revealed a problem with the specificity of the antibodies and conjugates used. Unfortunately because of time factors, further analysis of the linked antibodies, the conjugates and the successful linkage processes, could not be undertaken and many questions were thus left unanswered.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Additional Information: Adviser: Linda Walsh
• Keywords: Medicine
• Date of Award: 1993
• Depositing User: Enlighten Team
• Unique ID: glathesis:1993-74716
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 27 Sep 2019 17:00
• Last Modified: 27 Sep 2019 17:00
• URI: https://theses.gla.ac.uk/id/eprint/74716