Investigations of a Microbial Model for the Determination of Leakage of Root Canal Fillings

Chailertvanitkul, Pattama (1996) Investigations of a Microbial Model for the Determination of Leakage of Root Canal Fillings. PhD thesis, University of Glasgow.

Abstract

This study was a laboratory based investigation of a microbial model for the determination of leakage of root canal fillings using facultative anaerobes, obligate anaerobes, mixed micro-organisms and lipopolysaccharide as markers. Five hundred extracted human teeth with straight, single root canals were prepared chemo-mechanically and obturated with gutta-percha using different techniques and root canal sealers. These teeth were divided randomly into three groups for three major studies; the effect of smear layer on leakage, leakage immediately after obturation and also after long-term storage. A microbial model was developed. The cut end of a tube was sealed around the coronal part of each root canal so that the markers placed therein could move only through the obturated canal space. Each root was placed in a glass bottle containing sterile media and aliquots of the markers were placed in each coronal chamber. The model system was stored in the incubator at 37

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: William Philip Saunders
• Keywords: Medicine, Dentistry
• Date of Award: 1996
• Depositing User: Enlighten Team
• Unique ID: glathesis:1996-74754
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 27 Sep 2019 16:37
• Last Modified: 27 Sep 2019 16:37
• URI: https://theses.gla.ac.uk/id/eprint/74754