A P Element Excision-Derived Mutation in Drosophila melanogaster

Adam, Margaret M (1995) A P Element Excision-Derived Mutation in Drosophila melanogaster. PhD thesis, University of Glasgow.

Abstract

The purpose of the work described in this Thesis was to isolate and mutate novel genes essential in the development of the Drosophila melanogaster nervous system. Two marked P element enhancer trap strains were found to express the reporter gene lacZ in spatially and temporally regulated patterns in the nervous system. Excisions of the P element construct in one of these strains, A22, were detected in approximately 68% of the A22-derived progeny which were recognised by the loss of the rosy gene. 19 strains were subsequently found to have a recessive mutant phenotype believed to be a result of imprecise excision of the P element. This represents a mutation rate of approximately 24%. Each of these 19 strains had a recessive mutation that resulted in abnormal wing development. Other mutant phenotypes were not detected in any of the excision-derived strains. No similar mutations are known to be located at the site of P element insertion of the ancestral A22 strain and therefore these excision-derived mutants are considered novel. Four excision-derived mutants were selected for further characterisation and these were found to be cold-sensitive. The severity and frequency of the mutant phenotype was increased when these four strains were raised at 20

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Genetics
• Date of Award: 1995
• Depositing User: Enlighten Team
• Unique ID: glathesis:1995-74757
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 13 Nov 2019 15:58
• Last Modified: 13 Nov 2019 15:58
• URI: https://theses.gla.ac.uk/id/eprint/74757