A P Element Excision-Derived Mutation in Drosophila melanogaster

Adam, Margaret M (1995) A P Element Excision-Derived Mutation in Drosophila melanogaster. PhD thesis, University of Glasgow.

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The purpose of the work described in this Thesis was to isolate and mutate novel genes essential in the development of the Drosophila melanogaster nervous system. Two marked P element enhancer trap strains were found to express the reporter gene lacZ in spatially and temporally regulated patterns in the nervous system. Excisions of the P element construct in one of these strains, A22, were detected in approximately 68% of the A22-derived progeny which were recognised by the loss of the rosy gene. 19 strains were subsequently found to have a recessive mutant phenotype believed to be a result of imprecise excision of the P element. This represents a mutation rate of approximately 24%. Each of these 19 strains had a recessive mutation that resulted in abnormal wing development. Other mutant phenotypes were not detected in any of the excision-derived strains. No similar mutations are known to be located at the site of P element insertion of the ancestral A22 strain and therefore these excision-derived mutants are considered novel. Four excision-derived mutants were selected for further characterisation and these were found to be cold-sensitive. The severity and frequency of the mutant phenotype was increased when these four strains were raised at 20

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Genetics
Date of Award: 1995
Depositing User: Enlighten Team
Unique ID: glathesis:1995-74757
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 13 Nov 2019 15:58
Last Modified: 13 Nov 2019 15:58
URI: https://theses.gla.ac.uk/id/eprint/74757

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