Purification and Characterisation of the Herpes Simplex Virus Type 1 DNA Replication Protein UL8

Parry, Marc Edward (1993) Purification and Characterisation of the Herpes Simplex Virus Type 1 DNA Replication Protein UL8. PhD thesis, University of Glasgow.

Abstract

The genome of herpes simplex virus type 1 (HSV-1) contains seven genes whose products are directly involved in viral DNA replication. These are UL5, UL8, UL9, UL29, UL30, UL42 and UL52. I investigated the products of genes UL5, UL8, UL9, and UL52, as these were the least characterised. Peptides were synthesised with sequences corresponding to portions of the amino acid sequence of these proteins and the peptides were used to generate antisera in rabbits. The peptides produced sera reacting with two of the four proteins - UL8 and UL9. Sera were produced that reacted with both the N and C termini of UL8. The project now focused on UL8 protein which was purified to homogeneity to allow its further characterisation. An existing recombinant baculovirus/Spodoptera frugiperda expression system was used as a source of the protein. The protein was extracted from the cells in a soluble form and was stable in the extraction buffer (neither denatured nor proteolysed) for at least 4 hours at room temperature. The protein was also stable during freezing and thawing, dilution, and incubation at 4

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: Howard Marsden
• Keywords: Virology, Biochemistry
• Date of Award: 1993
• Depositing User: Enlighten Team
• Unique ID: glathesis:1993-74783
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 27 Sep 2019 16:31
• Last Modified: 27 Sep 2019 16:31
• URI: https://theses.gla.ac.uk/id/eprint/74783