Studies of Canine Acidophil Cell Hepatitis

Lindholm, Katarina Ingeborg Margaret (1993) Studies of Canine Acidophil Cell Hepatitis. PhD thesis, University of Glasgow.

Abstract

Canine Acidophil Cell Hepatitis (CACH), the subject of this study, was first described only relatively recently by Jarrett and O'Neil (1985). The aims of this study where to increase understanding of the disease, to investigate its experimental transmission to dogs and various laboratory animal species and to search for the aetiological agent. The introduction to this thesis describes the structure and function of normal and diseased liver, before concentrating on viruses known to cause liver disease in animals or humans and their resultant illnesses. A wide range are discussed since the aetiological agent of CACH has still to be isolated or identified. All of the protocols and techniques employed in this study are covered in the general materials and methods chapter. A sample of twenty CACH field cases described in chapter three, demonstrate the wide age range and variety of dogs which can be affected as well as the extensive geographical distribution of the disease throughout the country. The series was not a comprehensive coverage of all the CACH cases and suspected cases studied. Three canine transmission studies investigated the transmissibility of CACH, following the course of infection with frequent clinical, biochemical and haematological examinations. They provided material for other investigations including electron microscopy and serological studies. The last of the three used material from experimentally-infected rats to transmit CACH back to dogs. Transmissibility to various laboratory species was investigated, some were found to be more susceptible to CACH infection than others. Extensive electron microscopical examinations, of experim ental and field case material, located inumerable interesting particles but none could be definitely identified as virions. Preliminary serological studies, involving agar gel immunodiffusion (AGID) and peroxidaseantiperoxidase (PAP) immunocytochemistry, produced some interesting results but were not extensive enough to estimate the possibililty of developing a diagnostic test for use in living animals.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: William Jarrett
• Keywords: Veterinary science
• Date of Award: 1993
• Depositing User: Enlighten Team
• Unique ID: glathesis:1993-75520
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 19 Nov 2019 19:35
• Last Modified: 19 Nov 2019 19:35
• URI: https://theses.gla.ac.uk/id/eprint/75520