Characterisation of Glycoproteins in Populations of Globodera and Meloidogyne

Rakhit, Soma (1994) Characterisation of Glycoproteins in Populations of Globodera and Meloidogyne. MSc(R) thesis, University of Glasgow.

Abstract

This is a study into the surface glycoproteins and associated terminal sugar groups on the surface of the plant parasitic nematodes in Globodera spp., and Meloidogyne spp. The objective was to identify nematode surface glycoproteins and to investigate the possible role in the specific recognition of the suitable host plant by the parasite. In this study different populations of Meloidogyne spp (root knot nematode) and Globodera spp (potato cyst nematode) were studied using SDS polyacrylamide gel electrophoresis followed by western blotting, and the blots probed for the presence of glycoprotein using digoxygenin conjugated lectins which bind anti-digoxygenin antibody conjugated to alkaline phosphatase. Different populations of Meloidogyne spp (root knot nematode) and Globodera spp (potato cyst nematode) were compared using the above method. All eight populations of Meloidogyne tested contained >200 kDa proteins which bound Galanthus nivalis agglutinin (GNA lectin specific for mannose residues) strongly. The Globodera rostochiensis populations ROl and R05-88 could be distinguished by their differential staining of both general glycoprotein stain and peanut agglutinin lectin stain (specific for galactose residues). The G. pallida populations Luffness and Halton could not be differentiated from each other by any of the stains. Also GNA could differentiate five day old nematodes of population AR01 from eight to ten day old populations. The G. pallida population Luffness had a 60 kDa protein present in samples of nematode which had been hatched in tapwater only and not potato root diffusate. In addition a 150 kDa protein washed from the potato cyst nematode surface by the detergent CTAB may correspond to a family of proteins of molecular weight between 140 and 150 kDa detected on the glycoprotein stained western blot of the nematodes. Although more extensive testing is necessary using a wider variety of lectins and populations of nematodes, the differences in glycoprotein profiles of potato cyst nematodes may be useful in identification of nematode populations.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Additional Information: Adviser: Walter Robertson
• Keywords: Biochemistry
• Date of Award: 1994
• Depositing User: Enlighten Team
• Unique ID: glathesis:1994-75640
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 19 Nov 2019 19:02
• Last Modified: 19 Nov 2019 19:02
• URI: https://theses.gla.ac.uk/id/eprint/75640