Interactions of Herpes Simplex Virus Type-1 With the Cell Surface

Wood, Lisa J (1994) Interactions of Herpes Simplex Virus Type-1 With the Cell Surface. PhD thesis, University of Glasgow.

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The present study was concerned with the characterisation of the herpes simplex virus type 1 (HSV-1) UL25 gene product and analysis of the phenotype of the HSV-1 mutant tsl204 which has a temperature sensitive (ts) mutation in the UL25 gene. Previous work by Addison et al. (1984) suggested that tsl204 was unable to penetrate the cell membrane at the non-permissive temperature (NPT). This early defect could be overcome by brief incubation of mutant infected cells at the permissive temperature (PT). Upon further incubation of mutant-infected cells at the NPT, low numbers of intermediate capsids lacking DNA assembled in the nucleus. No full capsids were observed, indicating that tsl204 also had a defect in the production of virus particles. Although the work presented here confirmed that tsl204 has a defect in a step prior to the onset of viral protein synthesis, the data indicated that tsl204 had an uncoating defect at the NPT. A rabbit polyclonal antiserum raised against an oligopeptide representing anamino acid sequence present in the UL25 polypeptide was used to study the synthesis, processing and location of the UL25 gene product. The antiserum recognized a 67,000MW protein in virus-infected cell extracts and in purified virions, suggesting that the UL25 protein was a structural component of the virion. Using immunoprecipitation assays the UL25 gene product was detected at lh r post-infection in virus-infected cells at 37

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Additional Information: Adviser: John Subak-Sharpe
Keywords: Virology
Date of Award: 1994
Depositing User: Enlighten Team
Unique ID: glathesis:1994-75651
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 19 Nov 2019 19:00
Last Modified: 19 Nov 2019 19:00

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