Lysozyme-Small Molecule Interactions

Wadood, Abdul (1998) Lysozyme-Small Molecule Interactions. PhD thesis, University of Glasgow.

Abstract

The interaction of hen egg white lysozyme with penicillin and a range of metal ions has been studied using microcalorimetry and related biophysical techniques. UV difference and fluorescence experiments on the binding of penicillin to lysozyme are ambiguous and reflect the variety of published observations, but there is no clear evidence that penicillin competes for example with N-acetylglucosamine (NAG) in the binding cleft. This is supported by ITC binding experiments which suggest that penicillin-G binds to a site on lysozyme remote from the active site. DSC studies are complicated by the thermal instability of penicillin-G in solution above 35

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: Alan Cooper
• Keywords: Biochemistry, Molecular chemistry
• Date of Award: 1998
• Depositing User: Enlighten Team
• Unique ID: glathesis:1998-75896
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 19 Nov 2019 17:38
• Last Modified: 19 Nov 2019 17:38
• URI: https://theses.gla.ac.uk/id/eprint/75896