The Role of Glycoprotein C in Adsorption of Herpes Simplex Virus Type 1 (HSV-1)

Williams, Katherine (1999) The Role of Glycoprotein C in Adsorption of Herpes Simplex Virus Type 1 (HSV-1). MSc(R) thesis, University of Glasgow.

Abstract

The work presented in this thesis is a study of the role of gC in adsorption of herpes simplex virus type 1 (HSV-1). This study used several strains of wild type HSV-1 and their gC negative mutants strains 17, F, SC 16 and HFEM. In contrast to published results, comparison of the particle to p.f u ratios of each wild type strain to their gC negative form showed similar values, differing by two fold at most (Herold etal, 1991). Initially the ability of radioactively labelled strain 17 wild type and gC negative mutant virions to bind to BHK21/C13 and C6 gliomal cells was examined. There was no difference between either virus or between cell lines. Subsequently the adsorption kinetics of HSV-1 strain 17 wild type virus and its gC negative mutant were studied on BHK21/C13 cells. Again no differences were observed. Similarly a study of the rate of penetration of the HSV-1 strain 17 wild type and gC negative viruses demonstrated no difference in the rate of penetration into BHK21/C13 cells. The adsorption characteristics of the different strains and their gC negative mutants used in this study were compared on BHK21/C13 cells, with no difference being seen between any parental strains, and its gC negative mutant or between HSV-1 strain 17 wild type and any other. The adsorption kinetics of all strains and their gC negative mutants were compared on Vero cells with no difference being seen. beta-Galactosidase staining of lac Z expressing forms of some of the viral strains (HSV-1 strain 17, its gC negative mutant, SC16-DeltaUL44-Z and HFEM-DeltaUL44-Z) was used to examine adsorption kinetics on HeLa and 3T6 cells. All viruses displayed similar adsorption kinetics. In heparin inhibition assays all vimses demonstrated the same pattern of blocking by exogenous heparin although the levels of inhibition differed between strains. Experiments to determine the intracranial pathogenicity revealed no significant difference in pathogenicity with LD50 values of <101 and 5x101 for the wild type and mutant respectively. In peripheral pathogenicity and latency studies of the HSV-1 strain 17 viruses a ten fold difference was observed between the wild type and the mutant virus in data; this is not a significant difference. Reactivation frequency was also shown to be similar. The use of Southern and Western blotting confirmed the structure and gC expression of the HSV-1 strains and their mutants.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Additional Information: Adviser: Barklie Clements
• Keywords: Virology
• Date of Award: 1999
• Depositing User: Enlighten Team
• Unique ID: glathesis:1999-76209
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 19 Nov 2019 16:28
• Last Modified: 19 Nov 2019 16:28
• URI: https://theses.gla.ac.uk/id/eprint/76209