Stability function of plasmid ColK

Yaish, Sami Abdul-Rahman (1985) Stability function of plasmid ColK. MSc(R) thesis, University of Glasgow.

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ColK is a multicopy naturally occurring plasmid, that is very stably maintained in bacterial host cells. This work shows that the plasmid encodes a stability determinant designated ckr. that is involved in promoting site-specific recombination in multimeric plasmids, efficiently converting them to monomers. This apparently ensures stability by maximizing the number of segregating units at cell division. ckr has been located to a 0.37 kb fragment in ColK, its DNA sequence is highly homologous with that of cer (stability determinant of ColE1, a related plasmid to ColK). Recombination has been found to occur both between directly repeated cer copies and a direct repeat of one copy of cer and one of ckr and results in the deletion of the internal fragment between them. Inversion has been shown to occur between two cer copies in inverted repeat and in a substrate containing a copy of cer and a copy of ckr in inverted repeat. Both deletion and inversion are highly efficient.

Item Type: Thesis (MSc(R))
Qualification Level: Masters
Keywords: Genetics
Date of Award: 1985
Depositing User: Enlighten Team
Unique ID: glathesis:1985-76526
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 19 Nov 2019 14:13
Last Modified: 19 Nov 2019 14:13

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