AMP Deaminase From Saccharomyces cerevisiae

MacKenzie, Jane (1989) AMP Deaminase From Saccharomyces cerevisiae. PhD thesis, University of Glasgow.

Abstract

Attempts were made to clone the yeast adenosine-5'-monophosphate deaminase (AMP deaminase) gene from S. cerevisiae using a number of strategies. Initially, it was hoped that overproduction of AMP deaminase could be detected in yeast, by selecting for resistance to the AMP deaminase inhibitor, deoxycoformycin (dCF). This involved the construction of ade, hpt1 yeast strains in which the AMP deaminase reaction is the major source of inosine-5'-monophosphate (IMP). Although yeast AMP deaminase is sensitive to dCF in vitro no effect was observed in vivo (possibly due to lack of uptake of the drug).

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Genetics, Microbiology
• Date of Award: 1989
• Depositing User: Enlighten Team
• Unique ID: glathesis:1989-76911
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:28
• Last Modified: 14 Jan 2020 09:28
• URI: https://theses.gla.ac.uk/id/eprint/76911