Transcription in the Yeast Saccharomyces cerevisiae

Lithgow, Gordon James (1989) Transcription in the Yeast Saccharomyces cerevisiae. PhD thesis, University of Glasgow.

Abstract

The aim of this study was to investigate aspects of transcription of highly expressed yeast genes. To investigate the sequence requirements for transcriptional initiation putative promoters were constructed which contained promoter elements subcloned from the 5'-flanking region of the phosphoglycerate kinase gene (PGK1). The analysis of these promoters was hindered by high levels of transcriptional readthrough from adjacent sequences in the promoter probe vector. A CT-rich block, found upstream of many highly expressed yeast genes has previously been shown to influence the position of the transcriptional initiation site (I-site). Preliminary data presented here is consistent with the existence of a yeast protein which specifically interacts with this region.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Genetics, Microbiology
• Date of Award: 1989
• Depositing User: Enlighten Team
• Unique ID: glathesis:1989-76973
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:25
• Last Modified: 14 Jan 2020 09:25
• URI: https://theses.gla.ac.uk/id/eprint/76973