Sequence Analysis of Selected Regions of the Equine Herpesvirus 4 Genome

Riggio, Marcello Pasquale (1990) Sequence Analysis of Selected Regions of the Equine Herpesvirus 4 Genome. PhD thesis, University of Glasgow.

Abstract

The gene encoding glycoprotein gB of EHV-4 strain 1942 was located within a BamHI genomic library by a combination of dot blot and Southern blot hybridisation using probes derived from the HSV-1 gB DNA sequence, and its DNA sequence determined. The major portion of the gene mapped to a 2.9kb fragment at the left-hand end of BamHI-C with the transcriptional control domains and start of the coding sequences of the gene located at the right-hand end of BamHI-M. The unprocessed EHV-4 gB protein was predicted to be 975 amino acids (110kDa) in size and composed of an unusually long amino-terminal signal sequence, a hydrophilic surface domain containing 11 potential N-linked glycosylation sites and 10 cysteine residues, a hydrophobic transmembrane domain thought to span the membrane three times and a highly charged carboxyl-terminal cytoplasmic anchor domain. A potential internal proteolytic cleavage site was identified near the centre of the protein. The EHV-4 gB protein demonstrated high levels of conservation of primary amino acid sequence with other herpesvirus gB-like proteins. Furthermore, the conservation of all cysteine residues and some N-linked glycosylation sites in the surface domain between the gB-like proteins suggested possible conservation of secondary and tertiary structures. The gB-like proteins may have a similar, conserved function across the herpesviruses.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Veterinary science, Animal diseases
• Date of Award: 1990
• Depositing User: Enlighten Team
• Unique ID: glathesis:1990-77003
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:24
• Last Modified: 14 Jan 2020 09:24
• URI: https://theses.gla.ac.uk/id/eprint/77003