Transposition of Tn7

Morrell, Elizabeth (1990) Transposition of Tn7. PhD thesis, University of Glasgow.

Abstract

One of the transposition proteins of Tn7, TnsB, was overexpressed, and purified substantially. The protein has a subunit molecular weight of around 85 kDA, which is consistent with prediction from the DNA sequence, and appears to exist as a monomer in solution. The identity of the polypeptide was confirmed by N-terminal sequencing, which also demonstrated that the site of translational initiation is the first AUG in the reading frame, and that there is no N-terminal processing of the protein.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Genetics, Molecular biology
• Date of Award: 1990
• Depositing User: Enlighten Team
• Unique ID: glathesis:1990-77006
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:24
• Last Modified: 14 Jan 2020 09:24
• URI: https://theses.gla.ac.uk/id/eprint/77006